Key Responsibilities
Optimize protoplast isolation and regeneration protocols for efficient production of genome edited potato plants.
Gene construct development and validation.
Transient expression vectors producing CAS9 and gRNA, ribonucleoprotein (RNP) transfection, and virus-induced genome editing (VIGE) to achieve foreign-DNA-free edited events.
Molecular characterization of transgenic and genome edited events by PCR, RTq-PCR, Southern blot, sequencing and bioinformatics.
Phenotyping by artificial infection of plants using local pathogen strains in the biosafety glasshouse.
Able to cope with heavy intermittent workloads that might extend beyond office hours and during weekends.
Any other duties assigned.
Apply via :
cgiar.zohorecruit.com
